| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Freeze-drying Spermatozoa in Mice
凍結乾燥によるマウス精子の保存
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| Autores: |
鈴木, 宏志
川瀬, 洋介
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| Data: |
2008
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| Ano: |
2008
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| Palavras-chave: |
Mice
Freese-drying
Spermatozoa
マウス
凍結乾燥
精子
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| Resumo: |
Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.
セミナー「低温と乾燥による生物保存技術への挑戦～マミーエンジニアリング」3.
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| Idioma: |
Japonês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4510
info:doi/10.20585/cryobolcryotechnol.54.1_21
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| Editor: |
低温生物工学会
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| Direitos: |
低温生物工学会
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